Cytometry Systems

Standardization and Increased Efficiency in Sample Preparation

Cytometry Systems

Medimachine II

  • Touch Screen.
  • Special screen for lab gloves.
  • Manual and Automatic modes.
  • Multi-lingual settings.

Medimax

  • Tissue Disaggregation.
  • For use with or without enzymes.
  • Compact design easily.
  • Sterile and non-sterile applications.

Medimachine

  • Automated tissue dissociation.
  • Fat tissue preparation.
  • Improved reproducibility of cell suspension.
  • Closed disposable disaggregation unit.

Medicons & Filcons

  • Disbosable.
  • Sterile.
  • Non-Sterile.



  • Gentle Tissue Dissociation and Fine Homogenization

    Medimachine II System

    Medimachine II

    The Medimachine II is designed to provide both gentle tissue dissociation and fine homogenization using appropriate disposable devices with different technical features according to the required procedure.

    The instrument has an interactive touch-screen for selection of operation mode and for consulting the on board manual. This specific screen has been selected for use wearing lab gloves. The software offers multi-lingual settings and two modes: manual and automatic.

    The Medimachine II Dissociator is a benchtop instrument for the automated dissociation of tissues to obtain single-cell suspensions. Single-cell suspensions are a crucial prerequisite to achieve reliable results in cell separation, cell analysis, and cell culture. Moreover, the Medimachine II can be used for the homogenization of cells and tissues to extract biomolecules for molecular biology or protein biochemistry applications.
    Medimachine II homogenizator provides high quality results for extraction of molecules for the analysis of chemical residues, virus, bacterias, etc.


    Four types of disposables have been specially designed:

    • Medicons and Medicons Max
    • Prypcons and FOODcon7


    A number of Medimachine II programs have been specifically developed to offer standardized procedures for the gentle dissociation or fine homogenization of various samples. Operator could add and remove new customized programs.

    Specifications
    CE Certified Conform to UL / CSA
    Size 211 mm x 164 mm x 257 mm
    Weight 2.50 kg
    Power Consumption 60W
    Brochure
    Medimachine II System
    User Manual
    Medimachine II System

    For Automated, Tissue Dissociation

    Medimachine System

    Medimachine

    The Medimachine system is a sample preparation system for the automated, mechanical disaggregation of solid animal or plant tissues for flow cytometric analysis, cell culture, or DNA amplification.

    The Medimachine system consists of three components: the Medimachine, Medicons, and Filcons. Various pore sizes of disposable, sterile, and non-sterile Medicons and Filcons allow the operator to optimize experimental conditions to suit the tissue under study, ensuring clean, viable preparations of single cells, cell clusters, or cell nuclei. The procedure is fast and simple.

    An increasing number of laboratories are performing flow cytometric analyses of solid tissues and tumours for antigen expression, ploidy, and proliferative index. The quality of the final result in the flow cytometric analysis of any tissue depends upon the quality of the prepared suspension. Various manual methods for dissociating tissue have been used alone or in combination and include:

    • Chemical treatment
    • Manual disaggregation
    • Enzymatic digestion
    • Enucleation techniques

    These methods can be time consuming, and all have limitations with regard to operator safety and the quality of the final cell suspension. Tissue composition must be considered, since some tissues disaggregate more readily than others, and abnormal tissue might contain very delicate cells. Thus, differences in technicians’ skills and technique can result in low cell viability and yield, as well as poor reproducibility.

    Specifications
    Code: 79200
    Supply: 82/250 Vac 60/50 Hz. (switching)
    Absorption: 6W
    Weight: 2.5 Kg
    Dimensions: cm. 20 x 15 x 15
    Brochure
    Medimachine System
    Medimachine Workflow:
    Flexibility for different tissue types

    The following is an overview of the steps for using the Medimachine system for typical tissue disaggregation. The procedure should be adapted for your laboratory and each tissue type you receive. A small piece of tissue (up to 10 mm3), free of fat and necrosis, is inserted into the Medicons together with ~1.0 mL of the desired suspension buffer (for example, PBS).

    The Medicons is then inserted into the Medimachine and the machine is started. The machine can be run from 10 seconds to 4 minutes depending upon the tissue type and the cell suspension you want.

    As the tissue is disaggregated, the cells pass through the screen into the suspension liquid in the bottom of the Medicon (Figures 1 and 2).

    Once the tissue is disaggregated, the Medicons is removed from the Medimachine. A needless syringe is then inserted in the syringe port to recover the cell suspension. The Medicons may be rinsed with suspension buffer to ensure maximum cell recovery (Figure 3). Select the appropriate Filcons pore size for isolation of the desired cells (Figure 4).

     

    Medimax

    The Medimax system is a sample preparation system for the automated, mechanical disaggregation of solid animal or plant tissues for flow cytometric analysis, cell culture, or DNA amplification techniques.

    The Medimax system makes tissue preparation safe and independent of operator technique, and facilitates standardizing the preparation of all tissue types. Times and protocols can even be standardized across labs.

    The Medimax system consists of three components:

    • Medimax
    • Medicons
    • Filcons & Filcons Max

    The compact Medimax unit is the heart of the system. It works with all types of Medicons and operates at a constant speed of approximately 80 rpm.

    Specifications
    Code: 79210
    Supply: 82/250 Vac 60/50 Hz. (switching)
    Absorption: 6W
    Weight: 2.5 Kg
    Dimensions: cm. 22 x 25 x 20
    Brochure
    Medimax System

    A small piece of tissue (up to 10 mm3), free of fat and necrosis, is inserted into the Medicons together with the correct amount of liquid (~1.0 mL for codes 79300 and 79400; ~16.0 mL for code 79500) of the desired suspension buffer (for example, PBS)

    The Medicons is then inserted into the Medimax and the machine is started. The machine can be run from 10 seconds to 4 minutes depending upon the tissue type and the cell suspension you want. As the tissue is disaggregated, the cells pass through the screen into the suspension liquid in the bottom of the Medicons. Once the tissue is dissociated, the Medicons is removed from the Medimax. A needleless syringe is then inserted in the syringe port to recover the cell suspension. The Medicons may be rinsed with suspension buffer to ensure maximum cell recovery. Select the appropriate Filcons (or Filcons-Max if a 50 mL tube is used) pore size for isolation of the desired cells.

    Disposable Mechanical Disaggregator for Cell Dissociation

    Medicons & Medicons Max

    Medicons & Medicons Max

    Medicons are disposable polyethylene chambers containing an immobile stainless steel screen with approximately 100 hexagonal holes. Around each hole are six microblades designed for efficient cutting of hard and soft tissues

    Medicons
    Disposable disaggregator with 35µm or 50µm separator screens. H.D. Polyethylene casing. Sterile and non-sterile. The internal mechanical part is in stainless steel and is made up of a fixed screen with about 100 hexagonal bore-holes, around each of which there are 6 microblades. 1 ml liquid solution.



    Medicons Max
    Disposable disaggregator with 50µm separator screens. H.D. Polyethylene casing. Sterile and non-sterile. The internal mechanical part is in stainless steel and is made up of a fixed screen with about 250 hexagonal bore-holes, around each of which there are 6 microblades. 16 ml liquid solution.


    A stainless steel element rotates on the screen, bringing the tissue to the blades and letting the gently dissociated parts pass through the bore-holes. A micropump positioned under the screen makes sure that the liquid passes through the bore-holes, maintaining them constantly clean. Disaggregation of the sample takes from 30 seconds up to 4 minutes. Small quantities of tissue are sufficient because the recovery rate is very high.

    Specifications
    Medicons Code: 79300 SN / S - 79400 SN / S
    Medicons Max Code: 79500 SN - 79500 S
    A35 μm Recovery of cell sizes up to 160 μm
    50 μm Recovery of cell sizes up to 230 μm
    Brochure
    Medicons
    porous membrane
    Never place the tissue into a dry Medicons
    Do not overfill the Medicons with suspension buffer. This will cause the tissue to float and can result in inadequate disaggregation: dilution must be between 1 and 1,2 mL for standard Medicons (codes 79300 and 79400) and between 15 and 16 mL for Large Medicons (code 79500).
    If the amount of tissue is very small, the suspension buffer can be replaced with staining solution for maximum cell recovery.
    As the rotating element passes over the microblades, it tends to blunt the edges, reducing the cutting efficiency.
    Do not reuse the Medicons for successive samples.
    Large Medicons (big white) is available in 50 μm mesh size, while Medicons standard (green and orange) are available (sterile or not sterile) in two mesh sizes.
    *IVD and research use only - Not Medical devices

    Disposable Filter for Separating Cells and Nuclei

    Filcons Max

    Non Sterile 30 Micron Pore

    Orange Cup Type in package of 100 units

    Non Sterile 50 Micron Pore

    Green Cup Type in package of 100 units

    Non Sterile 70 Micron Pore

    Purple Cup Type in package of 100 units

    Non Sterile 100 Micron Pore

    Black Cup Type in package of 100 units

    Sterile 30 Micron Pore

    Orange Cup Type in package of 100 units

    Sterile 50 Micron Pore

    Green Cup Type in package of 100 units

    Sterile 70 Micron Pore

    Purple Cup Type in package of 100 units

    Sterile 100 Micron Pore

    Black Cup Type in package of 100 units

    Disposable Filter for Separating Cells and Nuclei

    Filcons & Filcons Max

    Filcons & Filcons Max

    Filcons
    A disposable filter for separating cells and nuclei. Its porous membrane has an effective surface area of 80 mm2, enabling large quantities of cell suspensions to be filtered.

    Filcons-Max
    A disposable filter for separating cells and nuclei. Its porous membrane has an effective surface area of 660 mm2, enabling large quantities of cell suspensions to be filtered.

    Filcons
    Filcons is a disposable filter for separating cells and nuclei. Its porous membrane has an effective surface area of 80 mm2, enabling large quantities of cell suspensions to be filtered. The microporous polyethylene funnel section is designed to pass the biological material to be retrieved while trapping waste particles under 1.5µm in size. Filcons filters must not be used more than once, as subsequent specimens could be contaminated. Before use, dampen the Filcons filter membrane with the cell or nuclei suspension medium. Filcons filters with syringe fittings can be used in either suction or compression. Maximum pressure is 25 psi (1.8 bar). Whichever method is employed, is recommended anyway to use the syringe as a reservoir without applying pressure to the plunger, so that the specimen is allowed to percolate through. For quantities of less than 5 ml the cup-type Filcons filter should be used.



    Filcons-Max
    Filcons-Max is a disposable filter for separating cells and nuclei. Its porous membrane has an effective surface area of 660 mm2, enabling large quantities of cell suspensions to be filtered. The microporous polyethylene funnel section is designed to pass the biological material to be retrieved while trapping waste particles under 1.5μm in size. Filcons-Max must not be used more than once, as subsequent specimens could be contaminated. Before use, dampen the filter membrane with the medium that is going to be used for filtration. Filcons-Max are only available as cup-type. They fit perfectly the 50 ml tubes as indicated in the pictures All packages contain 100 units. Non sterile Filcons-max are packed in 20 units/bag, Sterile FIlcons-Max are packed 1 unit/blister.

    Specifications
    Brochure
    Filcons Max

    Filcons
    After disaggregation of the tissue in the Medicons, the cell suspension is filtered using a Filcon disposable filter device. Depending upon the chosen Filcon pore size, cells or cell aggregates pass through, while larger particles are trapped. There are two types of Filcons: a syringe-fitting form which can be used with either suction or compression, which works well for quantities over 5 mL, and a cup type that works well for small quantities.

    Before use, wet the Filcons filter membrane with cell suspension buffer to reduce the surface tension and facilitate the filtration process.
    Isolation of nuclei, cells, and cell clusters can be achieved by varying the Filcons pore size.

    Filcons

    Non Sterile

    • Syringe Fitting
      100 pcs
    •  008-10N-S/1  - 10µm filter
    •  020-22N-S/1  - 20µm filter
    •  030-33N-S/1  - 30µm filter
    •  050-47N-S/1  - 50µm filter
    •  070-67N-S/1  - 70µm filter
    •  100-98N-S/1  - 100µm filter
    •  200-20N-S/1  - 200µm filter
    •  500-50N-S/1  - 500µm filter

    Non Sterile

    • Cup-Type
      100 pcs
    •  108-10N-S/1  - 10µm filter
    •  120-22N-S/1  - 20µm filter
    •  130-33N-S/1  - 30µm filter
    •  150-47N-S/1  - 50µm filter
    •  170-67N-S/1  - 70µm filter
    •  200-98N-S/1  - 100µm filter
    •  220-20N-S/1  - 200µm filter
    •  550-50N-S/1  - 500µm filter

    Non Sterile

    • Syringe Fitting
      300 pcs
    •  008-10N-S/3  - 10µm filter
    •  020-22N-S/3  - 20µm filter
    •  030-33N-S/3  - 30µm filter
    •  050-47N-S/3  - 50µm filter
    •  070-67N-S/3  - 70µm filter
    •  100-98N-S/3  - 100µm filter
    •  200-20N-S/3  - 200µm filter
    •  500-50N-S/3  - 500µm filter

    Non Sterile

    • Cup-Type
      300 pcs
    •  108-10N-S/3  - 10µm filter
    •  120-22N-S/3  - 20µm filter
    •  130-33N-S/3  - 30µm filter
    •  150-47N-S/3  - 50µm filter
    •  170-67N-S/3  - 70µm filter
    •  200-98N-S/3  - 100µm filter
    •  220-20N-S/3  - 200µm filter
    •  550-50N-S/3  - 500µm filter
     

    Sterile

    • Syringe Fitting
      100 pcs
    •  008-10S-S/1  - 10µm filter
    •  020-22S-S/1  - 20µm filter
    •  030-33S-S/1  - 30µm filter
    •  050-47S-S/1  - 50µm filter
    •  070-67S-S/1  - 70µm filter
    •  100-98S-S/1  - 100µm filter
    •  200-20S-S/1  - 200µm filter
    •  500-50S-S/1  - 500µm filter

    Sterile

    • Cup-Type
      100 pcs
    •  108-10S-S/1  - 10µm filter
    •  120-22S-S/1  - 20µm filter
    •  130-33S-S/1  - 30µm filter
    •  150-47S-S/1  - 50µm filter
    •  170-67S-S/1  - 70µm filter
    •  200-98S-S/1  - 100µm filter
    •  220-20S-S/1  - 200µm filter
    •  550-50S-S/1  - 500µm filter

    Sterile

    • Syringe Fitting
      300 pcs
    •  008-10S-S/3  - 10µm filter
    •  020-22S-S/3  - 20µm filter
    •  030-33S-S/3  - 30µm filter
    •  050-47S-S/3  - 50µm filter
    •  070-67S-S/3  - 70µm filter
    •  100-98S-S/3  - 100µm filter
    •  200-20S-S/3  - 200µm filter
    •  500-50S-S/3  - 500µm filter

    Sterile

    • Cup-Type
      300 pcs
    •  108-10S-S/3  - 10µm filter
    •  120-22S-S/3  - 20µm filter
    •  130-33S-S/3  - 30µm filter
    •  150-47S-S/3  - 50µm filter
    •  170-67S-S/3  - 70µm filter
    •  200-98S-S/3  - 100µm filter
    •  220-20S-S/3  - 200µm filter
    •  550-50S-S/3  - 500µm filter
     

    Filcons Max

    Non Sterile

    • Cup-Type
      100 pcs
    •  131M33N  - 30µm Porosity
    •  151M47N - 50µm Porosity
    •  171M67N  - 70µm Porosity
    •  201M98N  - 100µm Porosity

    Sterile

    • Cup-Type
      100 pcs
    •  131M33S  - 30µm Porosity
    •  151M47S - 50µm Porosity
    •  171M67S  - 70µm Porosity
    •  201M98S  - 100µm Porosity